- Other
- Microtubule Gliding-1984
- Ash1
-
Localization
of Ash1 mRNA to the bud tip of S. cerevisiae. Ash1 mRNA
(expressed from its native promoter) was labelled by an
amplified in situ hybridization protocol and then imaged
using deconvolution microscopy. The 3-D view in this movie
shows that Ash1 mRNA is found in particles that are concentrated
near the cell cortex. (pdf)
- Takizawa, Peter A., Sil, Anita, Swedlow, Jason
R., Herskowitz, Ira and Vale, Ronald D. (1997) Actin-dependent
localization of an RNA encoding a cell-fate determinant
in yeast. Nature 389: 90-93.
- in
vitro microtubule gliding
-
Kinesin,
expressed in bacteria, is absorbed onto the surface of a
glass slide. Rhodamine-labelled microtubules and ATP (1mM)
are introduced, and the adsorbed motors transport the microtubules
across the surface. Motors are randomly adsorbed, but only
motors that are oriented properly with respect to the microtubule
axis are able to produce motion.
- Single
molecule kinesin
-
Kinesin
fused to green fluorescence protein (S65T mutant) was expressed
in bacteria. Single molecule detection of the kinesin-GFP
was accomplished using a total internal reflection microscope.
The first sequence shows red microtubule tracks (cy5-labelled
sea urchin axonemes illuminated with a HeNe laser). The
next sequence shows individual kinesin molecules (green
spots)moving along these tracks. The kinesin molecules are
only clearly visible once they bind to the microtubule;
molecules in solution move rapidly by Brownian motion and
only produce a general background haze. In the third sequence
(black-and-white), the kinesin-GFP was translated in vitro
and added to the microtubules. Active, unidirectional motion
is seen along several axonemes.
- Dissociated
AxoPlasm-1983
- Movies of cytoskeletal
proteins in S-2 cells
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